Products
Recombinant Human EPO
- Product Name
- Recombinant Human EPO
- Catalogue No.
- P8174
- Species
- Human
- Host
- HEK293 cells
- Expression Region
- 28-193
- Mol. Weight
- 60-70 kDa
- Tags
- C-terminal Fc Tag
- Purity
- Greater than 95% as determined by SDS-PAGE
- Application
- Western Blot, ELISA
- Activity
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.03-0.1 units/mL.
- Formulation
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
- Reconstitution
- Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
- Storage
- The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
- Alternative Names
- EP Protein, EPO Protein, Epoetin Protein, erythropoietin Protein, MVCD2 Protein
- SDS-PAGE
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